Linlin Luo: Plant Physiology First Author

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Linlin Luo, first author of “TRANS-ACTING SIRNA3-derived short interfering RNAs confer cleavage of mRNAs in rice”

Current Position：

PhD candidate at Shenzhen University, Shenzhen, Guangdong province, China

Non-scientific Interests:

Reading, hiking, growing plants

Brief Bio:

I began my scientific research under Prof. Xuemei Chen’s supervision in Guangdong Provincial Key Laboratory for Plant Epigenetics from 2017. The researches of our laboratory are mainly on the epigenetic regulation of plants, with a focus on RNA modification, small RNA biosynthesis, degradation and modes of actions, as well as the molecular regulation mechanism of plant growth, development and stress responses. In this study, we systematically investigated the origin, evolution, target genes and potential biological functions of rice TRANS-ACTING SIRNA3 (TAS3)-derived siRNA (TAS3-siRNAs). Plant TAS3 siRNAs include tasiR-ARFs, which are functionally conserved in targeting AUXIN RESPONSE FACTOR (ARF) genes, and a set of non-tasiR-ARF siRNAs, which have rarely been studied. Using small RNA sequencing, we found that an overwhelming majority of TAS3 siRNAs belong to the non-tasiR-ARF group, while tasiR-ARFs occupy a diminutive fraction. Phylogenetic analysis of TAS3 genes across dicot and monocot plants revealed that the siRNA-generating regions were highly conserved in grass species, especially in the Oryzoideae. Target genes were identified for not only tasiR-ARFs but also non-tasiR-ARF siRNAs by analyzing rice Parallel Analysis of RNA Ends (PARE) datasets, and some of these siRNA-target interactions were experimentally confirmed using tas3 mutants generated by genome editing.

姓名：罗淋淋

目前职位：深圳大学，在读博士研究生

兴趣爱好：阅读、徒步、养花弄草

科研经历简介：

我于2017开始博士阶段的学习，师从著名学者陈雪梅教授。我们实验室主要研究植物的表观遗传调控，关注RNA修饰、非编码RNA的生物合成、降解及作用方式，以及其对植物生长发育、胁迫响应的分子调控机制研究。我所做的课题系统研究了水稻TAS3来源的siRNA（TAS3-siRNAs）的起源、进化、靶基因和潜在生物学功能。植物TAS3 siRNA包含2种：tasiR-ARF和non-tasiR-ARF siRNA，其中，tasiR-ARF的功能是保守的，然而non-tasiR-ARF siRNA的产生和功能却是未知的。水稻TAS3家族有5个成员，命名为TAS3a-e，我们通过构建单个TAS3成员突变体的小RNA文库，分析发现：TAS3来源的siRNA中，tasiR-ARF只占全部的2%，同时产生了大量的non-tasiR-ARF siRNA。进化树分析发现non-tasiR-ARF siRNA 从禾本科开始具有保守性；通过分析PARE数据库，我们预测了大量的靶基因，并且部分靶基因在突变体内得到了证实。