I see the light! Fluorescent proteins suitable for cell wall/apoplast targeting in Nicotiana benthamiana leaves (Plant Direct)

Imaging proteins through fluorescent tagging is tremendously powerful but also tricky. In this very useful paper, Stottard and Rolland survey the properties of 10 fluorescent tags to determine their effectiveness in the low pH environment of the plant cell wall and apoplast. The authors also test the propensity for the fluorescent proteins to aggregate, and whether or not they can be targeted to the apoplast. Finally, they describe parameters to consider when choosing a protein tag. A great read before you plan your next study! (Summary by Mary Williams) Plant Direct  10.1002/pld3.112