Review: Applications of plant volatile-mediated signaling
Plant Science Research Weekly, Research0 Comments
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Smell is one of the many senses that insects use to locate the plants that they use as food sources. Plants have evolved ways to exploit insects’ sense of smell, for example by producing volatile compounds that attract pollinators. Pickett and Khan review plant volatile–mediated signals in terms…
Review: Programmed Cell Death in Development and Disease
Plant Science Research Weekly, Research
Programmed cell death (PCD) is an active process that occurs as part of normal development and also contributes to defense against pathogens. While there are many similarities in developmental PCD (dPCD) and pathogen-triggered PCD (pPCD), there are also differences. Huysmans et al. review and contrast…
Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
Plant Science Research Weekly, ResearchSvitashev et al. demonstrate a new way to introduce CRISPR-Cas9 reagents into a plant cell, through the biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins. This approach was successful in the production of engineered plants, and also demonstrated that this method can be done in a DNA- and…
Improving carotenoid production in synthetic maize through data-driven mathematical modeling ($)
Plant Science Research Weekly, ResearchCarotenoids are nutritionally important phytonutrients. Comas et al. describe a strategy to enhance the production of cartotenoids in the seed endosperm. They start with quantitative metabolomics and gene expression data which they feed into mathematical models to determine which gene(s) need to be engineered.…
Review: Biotechnology and synthetic biology approaches for metabolic engineering of bioenergy crops
Plant Science Research Weekly, ResearchShih et al. review how the many and diverse tools of plant synthetic biology can be applied towards bioenergy crops, focusing on traits related to lignocellulose, oil, and soluble sugars. Tools include those that edit genes, those that alter protein activities, and those that enable gene stacking in…
Review: Plant synthetic biology for molecular engineering of signalling and development
Plant Science Research Weekly, ResearchNemhauser and Torii define synthetic biology as “an engineering approach to design, build and analyize dynamic molecular devices and/or pathways from biological components to produce cells and organisms with customized functionality.” In their review, they describe several plant synthetic biology…
Review: Plant synthetic promoters and transcription factors
Plant Science Research Weekly, ResearchMany plant traits are multigenic, so engineering them requires modulating the expression of several genes simultaneously. Synthetic promoters and transcription factors offer such a possibility. For example, a cis-element can be introduced into the promoter of each gene of interest, and a synthetic transcription…
Review: Multi-gene engineering in plants with RNA-guided Cas9 nuclease ($)
Plant Science Research Weekly, ResearchRaitskin and Patron review efforts to express multiple single guide RNA (sgRNAs) and Cas9 in plants for the coordinated expression of many genes. They argue for the need to create single plasmids carrying the sgRNAs and Cas9, using a Type IIS restriction endonuclease-mediated assembly method. Curr. Opin.…
Review: Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes
Plant Science Research Weekly, ResearchPuchta reviews the different ways that CRISPR/Cas9 can be used in synthetic biolgy, beyond the common gene-editing function. For example, the Cas9 protein can be fused to other proteins that activate or repress transcription, and targeted to different promoters using guide RNAs. He further explores the…