An efficient method of gene downregulation, where gene expression is reduced but not completely knocked out, is useful for crop improvement. Here, Shen et al. have developed a system to achieve this, by using CRISPR/Cas9 to insert an element containing a Kozak sequence and an ATG start codon just before the primary start codon of […]
Wheat (Triticum aestivum) is an important crop, making up ~20% of food consumed worldwide. As the population grows, wheat yields must increase to meet demand. To achieve this, genetically diverse wheat landraces (aka traditional varieties, which are locally adapted cultivars) can be utilized. Here, Cheng et al. whole-genome sequenced 827 wheat landraces from the Watkins […]
In mesophyll cells, carbonic anhydrase is mainly located in the chloroplast, however it is in the cytosol in plants with a C4 carbon concentrating mechanism. There is interest in relocating carbonic anhydrase to the cytosol of C3 plants as a first step in the introduction of a carbon concentrating mechanism. Here, Jethva et al. used […]
Photosynthesis is the process by which plants assimilate carbon by using light energy. However, with the solar energy conversion efficiency of many crop plants less than 1%, it is inefficient. Therefore, there is interest in manipulating photosynthesis for increased efficiency. Here, Croce et al. identifying nine strategies that have, or could be, implemented to achieve […]
Barley is a globally important cereal crop, so understanding barley grain development is of much interest. Here, Kovacik et al. investigated transcriptional changes in barley grains at five points across development, 4, 8, 16, 24 and 32 days after pollination. For each time point, grains were manually dissected into embryo, endosperm and seed maternal tissues, […]
Roots are important for acquiring water and nutrients from the soil; however root system architecture is poorly understood in cereal crops. Here Aldiss et al. used CRISPR/Cas9 to generate barley mutants in the auxin transporter PIN-FORMED2 (PIN2). Seedlings were grown in chambers and after four days the chambers were rotated by 90o. Whilst the roots […]
In the dark, plants modify thylakoid stacking to alter electron transport and reduce photodamage. More photosystem II (PSII) is located in thylakoids within stacked grana, which promotes cyclic electron transport. Upon light exposure, there is granal unstacking, which increases the amount of linear electron transport for carbon assimilation. Here Garty et al. used freeze fracture […]
Plants that use C4 photosynthesis, whereby carbon is concentrated around RuBisCo in bundle sheath cells, have high water and nitrogen use efficiencies. Thus, understanding how C4 photosynthesis evolves is of much interest. The Flaveria genus provides a unique opportunity for this, as it contains species that are classed as C3, C4, C4-like and C3-C4 intermediates. […]
Wheat is a major global crop, but its yields are severely compromised by drought, thus developing varieties with a higher drought tolerance is important. Emmer wheat (Triticum turgidum) is a wheat ancestor that contains more genetic diversity than cultivated varieties, so it can be used in genome wide association studies (GWAS) to discover alleles associated […]