Entries by Arif Ashraf

Integrated regulation of apical hook development by EIN3/EIL1 and PIFs ($) (Plant Cell)

The apical hook helps to protect the fragile cotyledons and shoot apical meristem while pushing through the soil, and hook angle determines the success of emergence from soil. Multiple hormones and light signals have contrasting roles in the regulation of hook formation; e.g., auxin, ethylene and gibberellic acid (GA) act as positive regulators whilst jasmonic […]

A separated vortex ring underlies the flight of the dandelion ($) (Nature)

Dandelions, perennial herbs from temperate zones, have the capacity to spread their seeds through wind for distances as far as 1 km. Ignazio Maria Viola and Naomi Nakayama with their team endeavored to understand this passive flight mechanism. They analyzed the flight mechanism of the dandelion by characterizing the fluid dynamics of the pappus (thread-like […]

CRY1 interacts with BES1 to regulate BR signaling and photomorphogenesis (Plant Cell)

Plants perceive blue light through cryptochrome (CRY) photoreceptors that are responsible for photomorphogenesis, flowering, and circadian rhythms. At the same time, brassinosteroid (BR) represses photomorphogenesis. In this article, Wang et al. demonstrated CRY1-mediated inhibition of BR signaling as a potential mechanism. In dark conditions, inactivated CRY1 does not interact with dephosphorylated BES1 (BRI1-EMS SUPPRESSOR1), a […]

Manipulation and sensing of auxin metabolism, transport and signaling ($) (Plant Cell Physiol)

Auxin is involved in a diverse array of developmental processes as well as biotic and abiotic stress responses. The chemical–genetic approach is a powerful tool to decipher the auxin signaling pathway. In this review, Fukui and Hayashi have provided a comprehensive overview about the structure, molecular targets, comparative activity and application of small molecules used […]

Agrobacterium tumefaciens enhances biosynthesis of two distinct auxins in crown galls ($) (Plant Cell Physiol.)

Kiyoshi et al. investigated auxin biosynthesis during the formation of crown galls by Agrobacterium tumefaciens. Agrobacterium introduces into the plant T-DNA containing two auxin biosynthesis genes iaaM (TRYPTOPHAN-2-MONOOXYGENASE) and iaaH (INDOLE-3-ACETAMIDE HYDROLASE). The authors used LC-MS/MS to show that the level of IAM (indole-3-acetamide), IAA (indole-3-acetic acid), PAM (phenylacetamide), PAA (phenylacetic acid) and related metabolites […]

CLASP sustains cell proliferation through a brassinosteroid signaling negative feedback loop ($) (Curr. Biol.)

Brassinostreroid (BR) regulates the development of the root apical meristem. High levels of BR have inhibitory effects on cell production rates in the meristem and low levels show the opposite effect. Ruan, Halat et al. showed that BR regulates microtubule-associated CLASP (CLIP-Associated Protein) expression through the transcription factors BZR1 and BES1 in a BRI1-dependent manner, […]

Review: Harnessing synthetic chemistry to probe and hijack auxin signaling (New Phytol)

Auxin has been studied since Charles Darwin observed the phototropic response. More recently, chemical genetic approaches using auxin agonists and antagonists have been applied to studies of auxin. Torii et al. review how synthetic chemistry and chemical genetics have provided insights into and new tools for controlling auxin signaling. The authors briefly discuss synthetic auxins […]

Epidermal expression of a sterol biosynthesis gene regulates root growth by a non-cell-autonomous mechanism in Arabidopsis (Development)

The HYDRA1 (HYD1) gene is primarily expressed in the root epidermis and encodes a sterol Δ8-Δ7 isomerase. The hyd1 mutant has defects in growth and radial patterning of root development. Short et al. showed that although HYD1 is not expressed in the vascular cells, PIN1 localization and vascular patterning are abnormal in the hyd1 mutant. […]