Single cell analysis of wheat spike development
Plant Science Research WeeklyThis preprint wins the award for “most beautiful paper” this week. Xu, Lin et al. carried out an expression analysis of developing wheat spikes at three developmental stages, using both single-cell RNA sequencing and single-molecule fluorescence in situ hybridization (smFISH). The wheat spike is…
Single cell multiomic analysis of plant immunity reveals PRIMER cells
Plant Science Research WeeklySingle cell mutiomics are radically changing our understanding of pretty much every cellular process. Here, Nobori et al. integrated single-cell transcriptomic, epigenomic and spatial transcriptomic data to investigate plant responses to pathogens. The authors used three different strains of Pseudomonas…
Single cell transcriptomics after mild drought reveals two types of mesophyll responses
Plant Science Research WeeklySingle-cell transcriptomics offer unprecedented insights into how plants respond to their environment. Although this technique is powerful, it requires extensive processing of the tissue (cell wall digestion to form protoplasts) prior to sequencing, which can induce additional transcriptional changes.…
New kid on the plant block: Single-cell proteomics
Plant Science Research WeeklyWhile single-cell omics technologies, particularly transcriptomics, are already becoming widely adopted in plant science, quantifying proteins at single cell resolution is less established. Fortunately, important technological strides have been made that improve sample preparation, separation techniques,…