Recognizing Plant Physiology first authors: Hongyu Wu
Hongyu Wu, first author of An efficient system for Ds transposon tagging in Brachypodium distachyon L.
Current Position: Postdoctoral fellow, State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, China
Education: PhD of Cell Biology (2018), State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong 271018, China
Non-scientific Interests: walking, painting, play badminton
Brief bio: I have great interests on plants. When I was an undergraduate student, I went to a lab to learn experimental skills on the tissue culture of Rhododendron. Later I’m quite lucky and joined Prof. Hailong An’s group at Shandong Agricultural University to pursue for PhD degree. At this stage, I started to work on Ac/Ds transposon tagging in Brachypodium distachyon. Then we established an efficient Ac/Ds transposon tagging system and population in B. distachyon. The Ac transposase cassette and Ds element were constructed in the same T-DNA and segregate in next generation. Based on G418 and GFP assays, the Ds-only plants could be isolated easily at an efficiency about 10% from the segregating progenies. So far the Ds population reached to a number of 710 and Ac transposase cassette only lines were obtained, which is a good resource for regional mutagenesis of any gene of interest in B. distachyon. In my current position, I will continue my research on Brachypodium and extend to the turf grasses.
姓名：武红玉
目前职位：山东农业大学，园艺科学与工程学院，博士后
教育背景：山东农业大学，生命科学学院，细胞生物学，理学博士
兴趣爱好：散步，绘画，乒乓球
简介：我对植物非常感兴趣，在本科阶段就申请进入实验室，参加了杜鹃花组织培养技术的研究。之后分别作为硕士和博士研究生在山东农业大学生命科学学院安海龙教授课题组从事二穗短柄草Ac/Ds转座体系及群体的研究。我们将Ac转座酶表达盒和Ds转座子构建在一个T-DNA上，当Ds发生转座后，在子代可以筛选获得仅含Ds不含T-DNA（Ac转座酶表达盒）的植株。基于G418抗性（T-DNA特异）和GFP荧光（Ds特异）分析，部分T-DNA株系能够达到10%左右的仅Ds植株筛选频率，迄今已获得旁邻序列已知的仅Ds独立株系710个，结合筛选获得的仅Ac转座酶表达盒株系，可以有效地在二穗短柄草中通过杂交方法导入Ac转座酶诱导这些Ds的再次转座，实现高效诱变和获得Ds旁邻基因的插入突变体，从而形成了一套有很高价值的二穗短柄草Ac/Ds转座体系和群体。我目前作为山东农业大学园艺科学与工程学院的博士后，主要依托二穗短柄草和草坪草开展功能基因克隆和作用机理研究。
