Recognizing Plant Physiology authors: Wei Shan

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Wei Shan, first author of MaXB3 Modulates MaNAC2, MaACS1 and MaACO1 Stability to Repress Ethylene Biosynthesis during Banana Fruit Ripening

Current Position: Lecturer, College of Horticulture, South China Agricultural University

Education: B. S., M.S. and D. S. in Agriculture from South China Agricultural University

Non-scientific Interests: running, movies, cooking

 Brief Bio: I did my masters and doctoral degrees in the lab of Prof. Jian-ye Chen and Prof. Wang-jin Lu, at South China Agricultural University, where I investigated the mechanism concerning NAC transcription factors (TFs) in regulation of banana ripening. At that time, we identified two banana NAC TFs, MaNAC1 and MaNAC2, which were found to be involved in fruit ripening through their interactions with ethylene signalling component MaEIL5. After graduation, I worked in South China Agricultural University and continued my research in the same group. Currently, I am studying the possible roles of E3 ubiquitin ligases in banana fruit ripening and quality formation. In a yeast two-hybrid screen using MaNAC2 as bait, we identified a RING-Type E3 Ligase MaXB3. Further experiments revealed that MaXB3 promoted the degradation of MaNAC2 through the ubiquitin-proteasome pathway, thus suppressing MaNAC2-mediated transcriptional repression on ripening-related genes. Additionally, MaXB3 ubiquitinated and degraded two key enzymes in ethylene biosynthesis, MaACS1 and MaACO1, to negatively regulate ethylene biosynthesis. Finally, we established a regulatory cascade involving MaXB3, MaNAC2, MaACS1 and MaACO1 that controls ethylene biosynthesis during banana ripening. Future direction may focus on the ubiquitination of TFs in more details, to further reveal the controlling mechanisms underlying banana fruit ripening at transcriptional and post-translational levels.

 

姓名：单伟

现在职位：华南农业大学，园艺学院，讲师

教育经历：2009-06至2016-06，硕士和博士研究生均就读于华南农业大学，导师均为陆旺金教授

兴趣爱好：跑步，电影，烹饪

个人简介：本人硕、博士就读于华南农业大学陆旺金教授和陈建业教授的实验室，从事NAC类转录因子调控香蕉果实采后成熟方面的研究。期间发现MaNAC1/2通过与乙烯信号通路关键转录因子MaEIL5互作，参与调控香蕉果实成熟。博士毕业后留校任教，在陆教授和陈教授的团队继续研究香蕉果实成熟过程中E3泛素连接酶及其介导的泛素化修饰的调控作用。以MaNAC2为钓饵，通过酵母双杂交筛库，我们筛选到一个E3泛素连接酶-MaXB3。MaXB3通过介导MaNAC2泛素化降解，抑制MaNAC2对下游成熟相关基因的调控。同时，MaXB3泛素化降解乙烯合成关键酶MaACS1和MaACO1，直接抑制乙烯合成。综上，我们揭示出MaXB3、MaNAC2、MaACS1和MaACO1形成调控乙烯合成的级联网络，参与香蕉果实成熟过程。后续，我们将继续以转录因子及其泛素化修饰为切入点，深入研究香蕉采后成熟与品质形成的转录调控机制和翻译后修饰机制。