Meng-Qi Feng: Plant Physiology First Author

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Meng-Qi Feng, first author of “miR171-targeted SCARECROW-LIKE genes CsSCL2 and CsSCL3 regulate somatic embryogenesis in citrus”

Current Position: Ph.D, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University

Education:

2016.09-2022.12 Huazhong Agricultural University, Ph.D.

2012.09-2016.06 Shandong Agricultural University, Bachelor

Non-scientific interests: badminton, swimming

Brief bio:

I have been studying for my PhD in Prof. Wen-Wu Guo’s Cell Engineering and Breeding Lab and conducting research on citrus somatic embryogenesis (SE) mechanism under the supervision of Prof. Guo and Dr. Xiao-Meng Wu, aiming at improving the efficiency of germplasm innovation in vitro. We use citrus embryonic callus as the material to elucidate the function and regulatory pathways of miRNA-target genes in SE by genetic transformation and biochemical experiments. In the working models we propose, miR156a-CsSPL regulate the expression of downstream miR172d-CsTOE, thus to alter starch accumulation level in callus and regulate SE in citrus; the target genes of miR171c, CsSCL2/3, modulate ROS homeostasis through the interactive protein CsClot and directly suppress the expression of regeneration-related

genes, thus regulating SE in citrus. The results have been published in Journal of Experimental Botany (2022) and Plant Physiology (2023).

论文：The miR171-targeted CsSCLs regulate somatic embryogenesis by modulating ROS homeostasis and regeneration-related genes

姓名：冯梦琦

目前职位：求职中

教育经历：

2016.09-2022.12 华中农业大学，博士

2012.09-2016.06 山东农业大学，学士

兴趣爱好：羽毛球，游泳

个人简介：

本人于2016年有幸加入郭文武教授的柑橘细胞工程育种课题组，在郭老师和伍小萌博士的指导下进行柑橘体细胞胚发生机理研究，旨在提高离体种质创新效率。我们以柑橘胚性愈伤组织为材料，通过遗传转化和生化实验等多种手段，解析miRNA-靶基因调控体细胞胚发生的功能和作用通路。提出miR156a-CsSPL调控下游miR172d-CsTOE表达，进而影响细胞内淀粉积累，从而控制愈伤组织体细胞胚发生的工作模型；提出miR171c靶基因CsSCL2/3通过互作蛋白CsClot降低细胞内ROS含量或直接抑制下游再生相关基因表达，从而调控体细胞胚发生的作用机理，研究结果发表于Journal of Experimental Botany (2022)，Plant Physiology (2023)。