Update: Advances in imaging plant cell dynamics

Since the days of Robert Hooke, microscopy has been one of the biologist’s most important tools, and continues to become ever more powerful. Komis et al. review recent advances in superresolution microscopy, particularly as they pertain to the dynamics of plant cells. Dynamic systems demand fast imaging, which can come at the expense of resolution. The authors discuss the applications and limitations key techniques including: structured illumination microscopy (SIM), photoactivation localization microscopy (PALM), stochastic optical reconstruction (STORM), stimulated emission depletion microscopy (STED), and light-sheet fluorescence microscopy (LSFM).  (Summary by Mary Williams) Plant Physiol. 10.1104/pp.17.00962